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SOLVED: The optimal annealing temperature (Ta) is the range of temperatures where efficiency of PCR amplification is maximal and depends directly On iength and composition of the primer(s) An annealing temperature 5"€
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Components Of A Typical PCR Reaction Mix PCR Reaction Buffer (usually supplied at 10X) 100mM Tris-HCl - Essentially a pH Buffer. Maintains appropriate. - ppt download
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